RESTRICTION FRAGMENT LENGTH POLYMORPHISM Meaning and
Definition
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Restriction Fragment Length Polymorphism (RFLP) is a molecular biology technique used to identify variations in DNA sequences among individuals or populations. It involves the digestion of DNA molecules with specific restriction enzymes, which are enzymes that recognize and cleave DNA at specific nucleotide sequences called restriction sites. The resulting DNA fragments are then separated by size using a technique called gel electrophoresis.
The variations in DNA sequences can result in differences in the restriction sites recognized by the restriction enzymes, leading to variations in the sizes of DNA fragments produced upon digestion. These variations in fragment lengths are referred to as polymorphisms.
RFLP analysis starts by extracting DNA from the cells or tissues of interest. The extracted DNA is then digested with restriction enzymes, which cut the DNA into smaller fragments at the recognition sites. After digestion, the mixture of DNA fragments is loaded onto a gel matrix and subjected to electrophoresis. During electrophoresis, an electric field is applied to the gel, causing the DNA fragments to migrate through the gel matrix. The smaller fragments migrate faster and cover longer distances, while the larger fragments migrate slower and cover shorter distances.
Once the gel electrophoresis is complete, the DNA fragments are visualized using various staining techniques or by Southern blotting, which involves transferring the DNA fragments to a membrane and hybridizing them with specific probes. The resulting patterns of DNA fragments on the gel or membrane can be compared among individuals or populations to determine differences or similarities in their DNA sequences. RFLP has been extensively used in genetic research, population genetics, forensics, and resolving paternity disputes.
Common Misspellings for RESTRICTION FRAGMENT LENGTH POLYMORPHISM
- eestriction fragment length polymorphism
- destriction fragment length polymorphism
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- testriction fragment length polymorphism
- 5estriction fragment length polymorphism
- 4estriction fragment length polymorphism
- rwstriction fragment length polymorphism
- rsstriction fragment length polymorphism
- rdstriction fragment length polymorphism
- rrstriction fragment length polymorphism
- r4striction fragment length polymorphism
- r3striction fragment length polymorphism
- reatriction fragment length polymorphism
- reztriction fragment length polymorphism
- rextriction fragment length polymorphism
- redtriction fragment length polymorphism
- reetriction fragment length polymorphism
- rewtriction fragment length polymorphism
- resrriction fragment length polymorphism
- resfriction fragment length polymorphism
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