POLY DT Meaning and
Definition
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Poly dT, also known as polydeoxythymidine or poly-T, is a synthetic polymer composed of repeating units of thymidine nucleotides. It is commonly used in molecular biology and genetics research as a tool to specifically bind to and isolate messenger RNA (mRNA) molecules for various applications.
Poly dT is predominantly used in techniques such as reverse transcription and cDNA synthesis, where it acts as a primer for the synthesis of complementary DNA (cDNA) from mRNA templates. It possesses a high affinity for the polyadenine (polyA) tail, a characteristic feature of most eukaryotic mRNA molecules. The interaction between the poly dT primer and the polyA tail allows for the initiation of reverse transcription, enabling the synthesis of cDNA molecules that are complementary to the mRNA sequences.
By incorporating a stretch of thymidine residues, poly dT is able to selectively interact with mRNA and exclude other RNA species, such as ribosomal RNA (rRNA) and transfer RNA (tRNA). This specificity is crucial for isolating and studying mRNA in order to gain insights into gene expression patterns or investigate the presence of specific RNA transcripts.
Overall, poly dT serves as a versatile reagent in molecular biology techniques, facilitating the analysis and manipulation of mRNA molecules for a wide range of scientific investigations.
Common Misspellings for POLY DT
- ooly dt
- loly dt
- -oly dt
- 0oly dt
- pily dt
- pkly dt
- plly dt
- pply dt
- p0ly dt
- p9ly dt
- poky dt
- popy dt
- pooy dt
- polt dt
- polg dt
- polh dt
- polu dt
- pol7 dt
- pol6 dt
- poly st
Etymology of POLY DT
The term "Poly dT" is a molecular biology abbreviation that refers to a polydeoxythymidine tract, specifically a chain of deoxythymidine nucleotides (T) in a DNA molecule.
In terms of its etymology, "Poly" is derived from the ancient Greek word "polus", meaning "many" or "multiple". In molecular biology, "Poly" is often used as a prefix to indicate a polymer, which refers to a large molecule made up of repeating subunits.
The "d" in "Poly dT" stands for "deoxy", which indicates that the DNA molecule contains deoxyribose as its sugar component, rather than ribose. "dT" represents the nucleotide thymidine in its deoxyribose form.
Overall, the term "Poly dT" simply denotes a chain of multiple thymidine nucleotides in DNA.
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